timp 1 concentration by elisa  (R&D Systems)

Journal: Journal of Inflammation Research

Article Title: TIMP4 as a Potential Complementary Biomarker and Therapeutic Target in Membranous Nephropathy: A Multi-Omics Investigation with Clinical Validation

doi: 10.2147/JIR.S545422

Figure Lengend Snippet: The results of Bayesian colocalization analysis. ( A ) TIMP4; ( B ) LY75. The figure illustrates meaningful colocalization results based on plasma proteins, with the instrumental variables and their chromosomal positions visualized.

Article Snippet: TIMP4 concentrations were quantified using a commercial enzyme-linked immunosorbent assay (ELISA) system (KE00198; Proteintech) according to the manufacturer’s protocols.

Techniques: Clinical Proteomics

Journal: Journal of Inflammation Research

Article Title: TIMP4 as a Potential Complementary Biomarker and Therapeutic Target in Membranous Nephropathy: A Multi-Omics Investigation with Clinical Validation

doi: 10.2147/JIR.S545422

Figure Lengend Snippet: Validation of TIMP4 protein expression. ( A ) TIMP4 levels in MN patients vs controls. ( B ) TIMP4 expression across PLA2R-positive MN, PLA2R-negative MN, and control groups. *** P < 0.001; ns, not significant ( P > 0.05).

Article Snippet: TIMP4 concentrations were quantified using a commercial enzyme-linked immunosorbent assay (ELISA) system (KE00198; Proteintech) according to the manufacturer’s protocols.

Techniques: Biomarker Discovery, Expressing, Control

Journal: Journal of Inflammation Research

Article Title: TIMP4 as a Potential Complementary Biomarker and Therapeutic Target in Membranous Nephropathy: A Multi-Omics Investigation with Clinical Validation

doi: 10.2147/JIR.S545422

Figure Lengend Snippet: Single-cell RNA sequencing profile of kidney tissues. ( A ) UMAP projection after Harmony batch correction, integrating datasets across samples. ( B ) Eighteen distinct clusters visualized by UMAP plotting. ( C ) Dot plot illustrating the expression of marker genes in different cell types. ( D ) Annotation of 13 major cell types based on marker genes. ( E ) Violin plot depicting TIMP4 expression levels across different cell populations.

Article Snippet: TIMP4 concentrations were quantified using a commercial enzyme-linked immunosorbent assay (ELISA) system (KE00198; Proteintech) according to the manufacturer’s protocols.

Techniques: RNA Sequencing, Expressing, Marker

Journal: Cancers

Article Title: TNF-α May Exert Different Antitumor Effects in Response to Radioactive Iodine Therapy in Papillary Thyroid Cancer with/without Autoimmune Thyroiditis

doi: 10.3390/cancers13143609

Figure Lengend Snippet: Biochemical parameters in the papillary thyroid cancer and papillary thyroid cancer associated with Hashimoto thyroiditis patients before and 4 days after radioiodine therapy.

Article Snippet: The ELISA kits required for the quantitative determination of serum MMP-9, TIMP-1, TNF-α, TNFR1 and TNFR2 concentrations were purchased from R&D Systems Inc. (Minneapolis, MN, USA).

Techniques:

Journal: Cancers

Article Title: TNF-α May Exert Different Antitumor Effects in Response to Radioactive Iodine Therapy in Papillary Thyroid Cancer with/without Autoimmune Thyroiditis

doi: 10.3390/cancers13143609

Figure Lengend Snippet: Correlations between MMP-9/TIMP-1 ratio and TNF-α/TNFR2 ratio measured before 131 I intake ( A ) and at 4 days after 131 I intake ( B ) in patients with papillary thyroid cancer associated with autoimmune thyroiditis.

Article Snippet: The ELISA kits required for the quantitative determination of serum MMP-9, TIMP-1, TNF-α, TNFR1 and TNFR2 concentrations were purchased from R&D Systems Inc. (Minneapolis, MN, USA).

Techniques:

Journal: Cancers

Article Title: TNF-α May Exert Different Antitumor Effects in Response to Radioactive Iodine Therapy in Papillary Thyroid Cancer with/without Autoimmune Thyroiditis

doi: 10.3390/cancers13143609

Figure Lengend Snippet: Correlations between MMP-9/TIMP-1 ratio and TNF-α/TNFR1 ratio ( A ) and TNF-α/TNFR2 ratio ( B ) measured at 4 days after 131 I intake in patients with papillary thyroid cancer.

Article Snippet: The ELISA kits required for the quantitative determination of serum MMP-9, TIMP-1, TNF-α, TNFR1 and TNFR2 concentrations were purchased from R&D Systems Inc. (Minneapolis, MN, USA).

Techniques:

Journal: Haematologica

Article Title: Endotoxemia shifts neutrophils with TIMP-free gelatinase B/MMP-9 from bone marrow to the periphery and induces systematic upregulation of TIMP-1

doi: 10.3324/haematol.2017.168799

Figure Lengend Snippet: Systematic induction of TIMP-1 and reduced bone marrow MMP gelatinolytic activity. (A) TIMP-1 and TIMP-2 protein content in bone marrow, spleen, lungs and liver of mice injected i.p. with LPS or control mice (PBS injection) as determined by ELISA. Protein levels detected by ELISA were corrected for the total protein concentration and are presented as nanograms of TIMP in one milligram of total protein. (B) Degradation of fluorogenic gelatin by gelatinases present in bone marrow samples from mice treated with LPS and control mice, in the presence or absence of an inhibitor of MMP-2 and MMP-9 (SB-3CT, 10 μM) or elastase inhibitor (ElaV, 10 μM). The velocity of the gelatin degradation reaction was expressed as fluorescence units (FU) per minute and is indicative of the net proteolytic activity present in the samples. Inhibition percentages are shown. * P <0.05, ** P <0.01, as determined by the Mann-Whitney test.

Article Snippet: TIMP-1 and TIMP-2 concentrations were determined using mouse TIMP-1 (DY980) and TIMP-2 (DY6304-05) DuoSet enzyme-linked immunosorbent assay (ELISA) kits (R&D Systems).

Techniques: Activity Assay, Injection, Enzyme-linked Immunosorbent Assay, Protein Concentration, Fluorescence, Inhibition, MANN-WHITNEY

Journal: Haematologica

Article Title: Endotoxemia shifts neutrophils with TIMP-free gelatinase B/MMP-9 from bone marrow to the periphery and induces systematic upregulation of TIMP-1

doi: 10.3324/haematol.2017.168799

Figure Lengend Snippet: Overview of the distribution pattern of MMP-9/TIMP-1 and MMP-2/TIMP-2 balances and MMP-9-positive cells upon challenge with lipopolysaccharide. Induction of endotoxemia results in the migration of MMP-9-positive polymorphonuclear cells out of the bone marrow to accumulate primarily in lungs and liver. This release of MMP-9 is meanwhile counteracted by a systematic upregulation of TIMP-1. In contrast, MMP-2 protein levels remain similar while TIMP-2 is generally reduced. NE; neutrophil elastase.

Article Snippet: TIMP-1 and TIMP-2 concentrations were determined using mouse TIMP-1 (DY980) and TIMP-2 (DY6304-05) DuoSet enzyme-linked immunosorbent assay (ELISA) kits (R&D Systems).

Techniques: Migration

Journal: PLoS ONE

Article Title: Low MMP-8/TIMP-1 reflects left ventricle impairment in takotsubo cardiomyopathy and high TIMP-1 may help to differentiate it from acute coronary syndrome

doi: 10.1371/journal.pone.0173371

Figure Lengend Snippet: Differences in acute-phase levels of A) matrix metalloproteinase-8 (MMP-8), B) tissue inhibitor of matrix metalloproteinase-1 (TIMP-1), and C) MMP-8/TIMP-1 molar ratio of acute coronary syndrome (ACS) (n = 2072) and takotsubo cardiomyopathy (TTC) (n = 45) compared to those of controls. D) Troponin T on admission and highest value during hospitalization in ACS and TTC. E) ROC curves of TIMP-1 and troponin T in differentiation of ACS from TTC. F) ROC curves after c-statistics showing the improved ability of TIMP-1 + other differing factors (age, sex and smoking) in differentiation between TTC and ACS over other differing factors alone . Data in box-plots are presented as medians, 25th and 75th percentiles (boxes), and 10th and 90th percentiles (whiskers).* p < 0.001; ** p = 0.01.

Article Snippet: Serum MMP-8 concentrations were determined by a time-resolved immunofluorometric assay (IFMA) and TIMP-1 concentrations by enzyme-linked immunosorbent assay (ELISA; R&D Systems, Minneapolis, MN, USA) from both study populations, as described [ ].

Techniques:

Journal: PLoS ONE

Article Title: Low MMP-8/TIMP-1 reflects left ventricle impairment in takotsubo cardiomyopathy and high TIMP-1 may help to differentiate it from acute coronary syndrome

doi: 10.1371/journal.pone.0173371

Figure Lengend Snippet: In TTC, MMP-8 and TIMP-1 levels also reflect ventricular impairment. Data in box-plots are presented as medians, 25th and 75th percentiles (boxes), and 10th and 90th percentiles (whiskers). * p = 0.01.

Article Snippet: Serum MMP-8 concentrations were determined by a time-resolved immunofluorometric assay (IFMA) and TIMP-1 concentrations by enzyme-linked immunosorbent assay (ELISA; R&D Systems, Minneapolis, MN, USA) from both study populations, as described [ ].

Techniques: